Bacteroides phages recovered from human and animal faecal matrices in rural Kenya

This data describes the recovering and isolation processes of Bacteroides spp. strains from human and cattle faecal sources from rural areas in Siaya County (Kenya), and occurred between 7th and 28th of June 2018. The data also includes the detection of bacteriophages (infecting these Bacteroides spp. host strains) in conjunction with traditional faecal indicator organisms in water sources from Kisumu and Siaya County (Kenya) occurring between June 18th 2018 and June 13th 2019. Exact location (coordinates) of the sample points are also described in the data set. A microbiological technique using Bile Esculin Bacteroides (BBE) agar was used for the recovering and isolation processes of Bacteroides spp. strains. Standard ISO (7899-2, 9308-1, 10705-2 and 10705-4) techniques, such as membrane filtration and the double-agar-layer methods, were used for the detection of bacteriophages and traditional faecal indicator organisms. The purpose of data collection was to develop new markers that could identify cattle and/or human sources of faecal contamination, which could be used as part of a Microbial Source Tracking (MST) tool box. Technicians and researchers from the University of Brighton (UK), University of Southampton (UK), from the Victoria Institute for Research on Environment and Development (VIRED) (KE) and from the Kenya Medical Research Institute (KEMRI) (KE) were responsible for the collection and interpretation of data. Full details about this dataset can be found at https://doi.org/10.5285/02c8a6b0-e59e-4278-b9a2-9958cd5a2c3c

Identification info

Metadata Language: English (en)

Character set: utf8

Dataset Reference Date (): 2019-12-06

Identifier: https://catalogue.ceh.ac.uk/id/02c8a6b0-e59e-4278-b9a2-9958cd5a2c3c

Identifier: doi: / 10.5285/02c8a6b0-e59e-4278-b9a2-9958cd5a2c3c

Other citation details: Trajano Gomes da Silva, D., Ebdon, J. , Mwangi, T., Okotto-okotto, J., Wright, J. (2019). Bacteroides phages recovered from human and animal faecal matrices in rural Kenya. NERC Environmental Information Data Centre 10.5285/02c8a6b0-e59e-4278-b9a2-9958cd5a2c3c

University of Brighton - Trajano Gomes da Silva, D.

University of Brighton - Ebdon, J.

Kenya Medical Research Institute - Mwangi, T.

Victoria Institute for Research on Environment and Development - Okotto-okotto, J.

University of Southampton - Wright, J.

NERC EDS Environmental Information Data Centre

University of Brighton - Trajano Gomes da Silva, D.

NERC Environmental Information Data Centre

Keywords

Water quality
Kenya
Microbial Source Tracking (MST)
Bacteroides
bacteriophages
Faecal Indicator Organisms (FIO)
drinking water sources

Limitations on Public Access: otherRestrictions

Other constraints: no limitations

Use constraints: otherRestrictions

Other constraints: This resource is available under the terms of the Open Government Licence

Use constraints: otherRestrictions

Other constraints: If you reuse this data, you should cite: Trajano Gomes da Silva, D., Ebdon, J. , Mwangi, T., Okotto-okotto, J., Wright, J. (2019). Bacteroides phages recovered from human and animal faecal matrices in rural Kenya. NERC Environmental Information Data Centre https://doi.org/10.5285/02c8a6b0-e59e-4278-b9a2-9958cd5a2c3c

Spatial representation type: textTable

Topic category

Environment

Begin date: 2018-06-01

End date: 2019-06-30

Code: WGS 84

Distribution Information

Data format

Comma-separated values (CSV) ()

Resource Locator: Download the data
Download a copy of this data

Resource Locator: Supporting information
Supporting information available to assist in re-use of this dataset

Resource Locator: Download the data
Download a copy of this data

Resource Locator: Supporting information
Supporting information available to assist in re-use of this dataset

Quality Scope: dataset

Other: dataset

Report

Dataset Reference Date (): 2010-12-08

Statement: The data came into existence after three steps: firstly a field collection of cattle and human faecal material for the isolation and recovering of Bacteroides spp. host strains ; secondly laboratory work were performed for the isolation of those strains; and thirdly field and laboratory work were done for assessing levels of bacteriophages and faecal indicator organism in environmental waters. Besides basic PPE (e.g.: gloves, glasses,..), fieldwork instrumentation included sterile wooden spatulas and an adapted sampling pole for the collection of cattle and human faeces from water pan shorelines and school pit latrines, respectively. Faecal and water samples were contained in sterile plastic containers in cooler bags containing ice packs and transported to KEMRI Kisian laboratory within 4 hours of collection. In KEMRI Kisian laboratory a total of 10 (5 from cattle and 5 from human faeces) Bacteroides spp. strains were recovered, isolated and preserved (frozen) for posterior use. These recently isolated Bacteroides spp. were then used as hosts, in the double-agar-layer method, for the detection of bacteriophages in Kenyan water sources. The units used for the detection of bacteriophages and traditional faecal indicator organism were plaque and /or colony forming units per 1 mL or 100 mL of water sample (PFU / 1 mL; PFU / 100 mL and CFU / 100 mL). Calibration of laboratory instrumentation as well as the use of blank samples (distilled water) were employed as data quality controls.

Metadata

File identifier: 02c8a6b0-e59e-4278-b9a2-9958cd5a2c3c XML

Metadata Language: English (en)

Character set: ISO/IEC 8859-1 (also known as Latin 1)

Resource type: dataset

Hierarchy level name: dataset

Metadata Date: 2022-05-18T12:37:40

Metadata standard name: UK GEMINI

Metadata standard version: 2.3

Environmental Information Data Centre

Lancaster Environment Centre, Library Avenue, Bailrigg , Lancaster , LA1 4AP , UK

Overviews

Publishing Body

Access the portal

Full access to the portal and metadata.

Associated resources (if any)

Not available

Default