16S rRNA genes of gut microbiota from calves in response to florfenicol

This data is NERC-funded but not held by the EIDC. This data is archived in the European Nucleotide Archive (ENA). This dataset contains sequences of 16S rRNA genes of gut microbiota of calves in response to preventive antibiotic therapy florfenicol by DNA-seq. The dataset was created from faecal samples (n=3) of the antibiotic treated animals over seven days and samples (n=3) of animals which have not been subjected to the antibiotic over the same time.

Identification info

Metadata Language: English (en)

Character set: utf8

Dataset Reference Date (): 2020-10-05

Identifier: https://catalogue.ceh.ac.uk/id/09f5d1fc-d850-4de1-8931-9f3599f6e65e

Coventry University - Igor Morozov

EIDC

European Nucleotide Archive

Keywords

florfenicol
preventive antibiotic treatment

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Topic category

Health
Farming

Distribution Information

Data format

FASTQ ()

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Dataset Reference Date (): 2010-12-08

Statement: The 16S rRNA genes from these samples were amplified and sequenced. Total DNA samples were sequenced at three time points: before treatment and at T3 (day 3) and T7 (day 7) after treatment. The V3 and V4 regions of the bacterial 16S rRNA gene were amplified with standard Illumina primers, forward primer: 5’-CCTACGGGNGGCWGCAG-3’ and reverse primer: 5’-GACTACHVGGGTATCTAATCC-3’ to produce 550 bp products for sequencing (Vertis, Germany). Sequencing was carried out on an Illumina MiSeq, producing 2x300bp paired-end reads.

Metadata

File identifier: 09f5d1fc-d850-4de1-8931-9f3599f6e65e XML

Metadata Language: English (en)

Character set: ISO/IEC 8859-1 (also known as Latin 1)

Resource type: dataset

Hierarchy level name: dataset

Metadata Date: 2020-10-05T15:54:52

Metadata standard name: UK GEMINI

Metadata standard version: 2.3

Environmental Information Data Centre

Lancaster Environment Centre, Library Avenue, Bailrigg , Lancaster , LA1 4AP , UK

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