Operational taxonomic units for epilithon bacteria in the rivers Wolf and Tamar catchments

This dataset contains operational taxonomic units for epilithon (water samples): Approximate location of sampling sites was determined from maps to provide good spatial coverage of the Wold River through to the Tamar River. Exact sites were determined in the field, considering accessibility and other logistics. The exact location of each sample site was determined using a Garmin GPS12. Three stones were taken from each of the 20 locations and epilithon removed from a defined area. Samples were kept in the cold and removed to the laboratory for analyses. DNA was extracted from all soil and epilithon samples using the MOBIO Powersoil 96 well DNA extraction kit. DNA was quality checked for purity and yield prior to submission for 454 pyrosequencing to assess both bacterial and eukaryotic biodiversity within each sample. Following bioinformatic sequence processing, sequencing were clustered into operational taxonomic units (OTU) and the data tables display the percentage of each OTU within each sample. Full details about this dataset can be found at https://doi.org/10.5285/16649ff0-af24-41b0-bcb4-15e610dac170

Identification info

Metadata Language: English (en)

Character set: utf8

Dataset Reference Date (): 2014-05-14

Dataset Reference Date (): 2010-01-01

Identifier: CEH:EIDC: / 1395928899368

Identifier: https://catalogue.ceh.ac.uk/id/16649ff0-af24-41b0-bcb4-15e610dac170

Identifier: doi: / 10.5285/16649ff0-af24-41b0-bcb4-15e610dac170

Other citation details: Griffiths, R., Edwards, F., Scarlett, P., Webb, G., Sarlej, R., Thomson, B. (2014). Operational taxonomic units for epilithon bacteria in the rivers Wolf and Tamar catchments. NERC Environmental Information Data Centre 10.5285/16649ff0-af24-41b0-bcb4-15e610dac170

UK Centre for Ecology & Hydrology - Griffiths, R.

Centre for Ecology & Hydrology - Griffiths, R.

Centre for Ecology & Hydrology - Edwards, F.

Centre for Ecology & Hydrology - Scarlett, P.

Centre for Ecology & Hydrology - Webb, G.

Centre for Ecology & Hydrology - Sarlej, R.

Centre for Ecology & Hydrology - Thomson, B.

NERC Environmental Information Data Centre

NERC EDS Environmental Information Data Centre

UK Centre for Ecology & Hydrology

Maintenance and update frequency: unknown

Maintenance note: not planned

GEMET - INSPIRE themes, version 1.0 ()

Land Use

Keywords

Soil
Biodiversity
Reynard Section
Soil
Environmental monitoring facilities
Species distribution
Hydrography
microbiology
genetic information
Tellus
South West project

Limitations on Public Access: otherRestrictions

Other constraints: no limitations

Use constraints: otherRestrictions

Other constraints: This resource is made available under the terms of the Open Government Licence

Use constraints: otherRestrictions

Other constraints: © UK Centre for Ecology & Hydrology

Use constraints: otherRestrictions

Other constraints: If you reuse this data, you should cite: Griffiths, R., Edwards, F., Scarlett, P., Webb, G., Sarlej, R., Thomson, B. (2014). Operational taxonomic units for epilithon bacteria in the rivers Wolf and Tamar catchments. NERC Environmental Information Data Centre https://doi.org/10.5285/16649ff0-af24-41b0-bcb4-15e610dac170

Spatial representation type: textTable

Topic category

Biota
Environment
Inland waters
Location

Begin date: 2013-10-01

End date: 2014-03-20

Code: OSGB 1936 / British National Grid

Distribution Information

Data format

Comma-separated values (CSV) ()

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Resource Locator: Supporting Information
Supporting information available to assist in re-use of this dataset

Quality Scope: dataset

Other: dataset

Report

Dataset Reference Date (): 2010-12-08

Statement: Epilithon (water samples): Approximate location of sampling sites was determined from maps to provide good spatial coverage of the Wold River through to the Tamar River. Exact sites were determined in the field, considering accessibility and other logistics. The exact location of each sample site was determined using a Garmin GPS12. Three stones were taken from each of the 20 locations and epilithon removed from a defined area. Samples were kept in the cold and removed to the laboratory for analyses. DNA was extracted from all soil and epilithon samples using the MOBIO Powersoil 96 well DNA extraction kit. DNA was quality checked for purity and yield prior to submission for 454 pyrosequencing to assess both bacterial and eukaryotic biodiversity within each sample. Following bioinformatic sequence processing, sequencing were clustered into operational taxonomic units (OTU) and the data tables display the percentage of each OTU within each sample.

Metadata

File identifier: 16649ff0-af24-41b0-bcb4-15e610dac170 XML

Metadata Language: English (en)

Character set: ISO/IEC 8859-1 (also known as Latin 1)

Resource type: dataset

Hierarchy level name: dataset

Metadata Date: 2021-06-25T18:44:50

Metadata standard name: UK GEMINI

Metadata standard version: 2.3

Environmental Information Data Centre

Lancaster Environment Centre, Library Avenue, Bailrigg , Lancaster , LA1 4AP , UK

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