Genotype data for three freshwater macroinvertebrate species in upland Wales, UK (2012-2013)

The resource consists of genotype data (genetic constitution of an individual organism) for three freshwater macroinvertebrate species, Amphinemura sulcicollis, Isoperla grammatica and Baetis rhodani collected at sites in upland Wales during May 2012 and May 2013. New nucleotide tandem repeats in DNA sequences i.e. Novel microsatellites were developed for this project so that genetic analysis could be carried out within the three species. Extra data on how microsatellite scores were grouped i.e. binned into unique alleles is provided, to aid replication. The data was generated for Hannah Macdonald's PhD thesis: to study genetic structure and diversity, demographic species history and resilience in freshwater ecosystems.

The work was carried out under Diversity in Upland Rivers for Ecosystem Service Sustainability (DURESS) project (Grant reference NERC NE/J014818/1). DURESS was funded by the Natural Environment Research Council (NERC) under the Biodiversity and Ecosystem Service Sustainability (BESS) programme. Full details about this dataset can be found at https://doi.org/10.5285/27da4400-9835-4241-9df9-a3b598f8d274

Identification info

Metadata Language: English (en)

Character set: utf8

Dataset Reference Date (): 2017-09-20

Dataset Reference Date (): 2013-06-15

Identifier: https://catalogue.ceh.ac.uk/id/27da4400-9835-4241-9df9-a3b598f8d274

Identifier: doi: / 10.5285/27da4400-9835-4241-9df9-a3b598f8d274

Other citation details: MacDonald, H.C., Bruford, M.W., Ormerod, S., Jones, T.H. (2017). Genotype data for three freshwater macroinvertebrate species in upland Wales, UK (2012-2013). NERC Environmental Information Data Centre 10.5285/27da4400-9835-4241-9df9-a3b598f8d274

Cardiff University - Hannah MacDonald

Cardiff University - MacDonald, H.C.

Cardiff University - Bruford, M.W.

Cardiff University - Ormerod, S.

Cardiff University - Jones, T.H.

NERC EDS Environmental Information Data Centre

NERC Environmental Information Data Centre

Cardiff University

Maintenance and update frequency: notPlanned

GEMET - INSPIRE themes, version 1.0 ()

Habitats and Biotopes

Wikidata

Keywords

Diversity of Upland Rivers for Ecosystem Service Sustainability (DURESS)
Microsatellites
Fragment analysis
Genetic diversity
Freshwater invertebrates

Limitations on Public Access: otherRestrictions

Other constraints: no limitations

Use constraints: otherRestrictions

Other constraints: This resource is made available under the terms of the Open Government Licence

Use constraints: otherRestrictions

Other constraints: © Cardiff University

Use constraints: otherRestrictions

Other constraints: If you reuse this data, you should cite: MacDonald, H.C., Bruford, M.W., Ormerod, S., Jones, T.H. (2017). Genotype data for three freshwater macroinvertebrate species in upland Wales, UK (2012-2013). NERC Environmental Information Data Centre https://doi.org/10.5285/27da4400-9835-4241-9df9-a3b598f8d274

Spatial representation type: textTable

Topic category

Biota

Begin date: 2012-06-15

End date: 2013-06-15

Code: OSGB 1936 / British National Grid

Distribution Information

Data format

Comma-separated values (CSV) ()

Resource Locator: Download the data
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Resource Locator: Supporting information
Supporting information available to assist in re-use of this dataset

Resource Locator: Download the data
Download a copy of this data

Resource Locator: Supporting information
Supporting information available to assist in re-use of this dataset

Quality Scope: dataset

Other: dataset

Report

Dataset Reference Date (): 2010-12-08

Statement: Samples of each invertebrate were collected by kicksampling in May in 2012 and 2013, samples were sorted and identified to genus level on site and stored in absolute ethanol. In the laboratory each larva was identified to species level using Elliott et al. (1988) and Hynes (1977). DNA was then extracted from tissue samples (see supporting information for extraction methods). Baetis rhodani samples contained cryptic species which had to be removed before genotyping, no cryptic species were identified for Amphinemura sulcicollis or Isoperla grammatica. Samples were sent for fragment analysis (Dundee Biosciences, UK) using ROX500 as the internal size standard. Fragment analysis products were visualised and scored using Genemarker v 1.91 (Holland and Parson 2011). These results were downloaded from Genemarker and allele sizes were binned by hand using the 'Binning' information (provided in the metadata) in an Excel spreadsheet. The data were then complied into master Excel spreadsheets for each invertebrate. The data were then exported as comma separated value files for ingestion into the EIDC.

Metadata

File identifier: 27da4400-9835-4241-9df9-a3b598f8d274 XML

Metadata Language: English (en)

Character set: ISO/IEC 8859-1 (also known as Latin 1)

Resource type: dataset

Hierarchy level name: dataset

Metadata Date: 2022-05-20T10:39:54

Metadata standard name: UK GEMINI

Metadata standard version: 2.3

Environmental Information Data Centre

Lancaster Environment Centre, Library Avenue, Bailrigg , Lancaster , LA1 4AP , UK

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