Heterogeneity and environmental stress resistance of wild yeast isolates

This dataset contains analytical results for wild yeast isolates from soil samples at and near a disused metal smelting works in the north-east of the UK. The main contaminant from the smelting works was lead (Pb), but Cd was also elevated. The soil near the smelting works showed above-background levels of Pb. Yeasts of similar colony morphology on laboratory agar were isolated and proved to be mostly isolates of Saitozyma podzolica: 56 independent clonal isolates of this yeast were confirmed by ITS sequencing and RAPD-PCR. All of the S. podzolica isolates were tested for Pb and Cd resistance (IC50) and heteroresistance. Heteroresistance indicated variation in metal resistance between individual cells of clonal populations. Some isolates were also tested with Zn. The genomes of the isolates were sequenced and sequence data submitted to the NCBI/EBI. Full details about this dataset can be found at https://doi.org/10.5285/

Identification info

Metadata Language: English (en)

Character set: utf8

Dataset Reference Date (): 2019-10-10

Identifier: https://catalogue.ceh.ac.uk/id/299f89cd-7bbe-4428-9552-c51981c5f883

Identifier: doi: / 10.5285/

Other citation details: Hewitt, S., Warry, A., Dyer, P.S., Avery, S. (2019). Heterogeneity and environmental stress resistance of wild yeast isolates. NERC EDS Environmental Information Data Centre 10.5285/

University of Nottingham - Simon Avery

University of Nottingham - Hewitt, S.

University of Nottingham - Warry, A.

University of Nottingham - Dyer, P.S.

University of Nottingham - Avery, S.

NERC EDS Environmental Information Data Centre

GEMET - INSPIRE themes, version 1.0 ()

Environmental Monitoring Facilities

Keywords

Environmental stress resistance
Wild Yeast
Heterogeneity

Limitations on Public Access: otherRestrictions

Other constraints: no limitations

Use constraints: otherRestrictions

Other constraints: This resource is available under the terms of the Open Government Licence

Use constraints: otherRestrictions

Other constraints: If you reuse this data, you should cite: Hewitt, S., Warry, A., Dyer, P.S., Avery, S. (2019). Heterogeneity and environmental stress resistance of wild yeast isolates. NERC EDS Environmental Information Data Centre https://doi.org/10.5285/

Spatial representation type: textTable

Topic category

Environment

Code: OSGB 1936 / British National Grid

Distribution Information

Data format

Comma-separated values (CSV) ()

Resource Locator: Download the data
Download a copy of this data

Resource Locator: Supporting information
Supporting information available to assist in re-use of this dataset

Resource Locator: Download the data
Download a copy of this data

Resource Locator: Supporting information
Supporting information available to assist in re-use of this dataset

Quality Scope: dataset

Other: dataset

Report

Dataset Reference Date (): 2010-12-08

Statement: Soil-core samples were collected during July 2014 near the disused St. Anthony’s metal smelting works, at either contaminated (16.03 g kg-1 Pb, 10.87 mg Kg-1 Cd) or nearby less-contaminated (4.38 g kg-1 Pb, 1.49 mg Kg-1 Cd) areas. Coordinates and a description of the site are available at http://www.twsitelines.info/SMR/4192. Soil-core samples were diluted in the laboratory and spread to agar media for yeast isolation. Single colonies were subcultured and yeasts identified with ITS sequencing and RAPD PCR. 56 clonal isolates of Saitozyma podzolica were assayed for lead (Pb) and cadmium (Cd) resistance (IC50) and heteroresistance. Some isolates were also tested with zinc (Zn) Genomic DNA preparations submitted to NBAF-Liverpool for whole-genome sequencing. The sequence data were deposited with EBI/NCBI.

Metadata

File identifier: 299f89cd-7bbe-4428-9552-c51981c5f883 XML

Metadata Language: English (en)

Character set: ISO/IEC 8859-1 (also known as Latin 1)

Resource type: dataset

Hierarchy level name: dataset

Metadata Date: 2022-05-05T17:32:00

Metadata standard name: UK GEMINI

Metadata standard version: 2.3

Environmental Information Data Centre

Lancaster Environment Centre, Library Avenue, Bailrigg , Lancaster , LA1 4AP , UK

Overviews

Publishing Body

Access the portal

Full access to the portal and metadata.

Associated resources (if any)

Not available

Default