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Time series of microbial carbon release from soil as carbon dioxide under different nitrogen and phosphorus treatments with a high molecular weight substrate added as a carbon source, Conwy catchment, North Wales, UK (2016)

Time series data of carbon release in disintegrations per minute are presented for different nitrogen and phosphorus treatments with a high molecular weight substrate added as a carbon source to soil samples from six depths (0-15, 15-30, 50-100, 100-150, 150-200 and 250-300 centimetres). Soil cores were collected from a field experiment in the Conwy catchment in July 2016 and returned the laboratories of the School of Environment, Natural Resources and Geography, Bangor University. A high molecular weight substrate was added as a carbon source to the samples and the rate of 14C-substrate mineralization measured.

All the work was carried out by trained members of staff from Bangor University and the Centre for Ecology & Hydrology.

The measurements were taken to improve understanding of the relationship between microbial activity and soil properties and depth of sampling, under differing nutrient availability.

The data were collected for the NERC project 'The Multi-Scale Response of Water quality, Biodiversity and Carbon Sequestration to Coupled Macronutrient Cycling from Source to Sea' (NE/J011991/1). The project is also referred to as Turf2Surf. Full details about this dataset can be found at https://doi.org/10.5285/4a8fedf4-a572-4d95-ac41-087974169af1

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Identification info

Metadata Language
English (en)
Character set
utf8
Dataset Reference Date ()
2018-03-20
Dataset Reference Date ()
2016-11-30
Identifier
https://catalogue.ceh.ac.uk/id/4a8fedf4-a572-4d95-ac41-087974169af1
Identifier
doi: / 10.5285/4a8fedf4-a572-4d95-ac41-087974169af1
Other citation details
Glanville, H.C., De Sosa, L.L. , Marshall, M.R., Cooper, D.M., Jones, D.L. (2018). Time series of microbial carbon release from soil as carbon dioxide under different nitrogen and phosphorus treatments with a high molecular weight substrate added as a carbon source, Conwy catchment, North Wales, UK (2016). NERC Environmental Information Data Centre 10.5285/4a8fedf4-a572-4d95-ac41-087974169af1
  Bangor University - Glanville, H.C.
  Bangor University - De Sosa, L.L.
  Centre for Ecology & Hydrology - Marshall, M.R.
  Centre for Ecology & Hydrology - Cooper, D.M.
  Bangor University - Jones, D.L.
  NERC EDS Environmental Information Data Centre
  NERC Environmental Information Data Centre
  Bangor University - Dr. Helen Glanville
Maintenance and update frequency
notPlanned
GEMET - INSPIRE themes, version 1.0 ()
  • Environmental Monitoring Facilities
Keywords
  • Environmental survey
  • T2S
  • Turf2Surf
  • Macronutrient Cycling Programme
  • MCP
  • North Wales
  • Conwy catchment
  • liquid scintillation counter
  • Soil Core
Limitations on Public Access
otherRestrictions
Other constraints
no limitations
Use constraints
otherRestrictions
Other constraints
This resource is made available under the terms of the Open Government Licence
Use constraints
otherRestrictions
Other constraints
© UK Centre for Ecology & Hydrology
Use constraints
otherRestrictions
Other constraints
© Bangor University
Use constraints
otherRestrictions
Other constraints
If you reuse this data, you should cite: Glanville, H.C., De Sosa, L.L. , Marshall, M.R., Cooper, D.M., Jones, D.L. (2018). Time series of microbial carbon release from soil as carbon dioxide under different nitrogen and phosphorus treatments with a high molecular weight substrate added as a carbon source, Conwy catchment, North Wales, UK (2016). NERC Environmental Information Data Centre https://doi.org/10.5285/4a8fedf4-a572-4d95-ac41-087974169af1
Spatial representation type
textTable
Distance
10  urn:ogc:def:uom:EPSG::9001
Topic category
  • Environment
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W


Begin date
2016-07-15
End date
2016-09-30
 
Code
WGS 84

Distribution Information

Data format
  • Comma-separated values (CSV) ()

Resource Locator
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Quality Scope
dataset
Other
dataset

Report

Dataset Reference Date ()
2010-12-08
Statement

Soil cores were collected from the field site and returned to laboratories of the School of Environment, Natural Resources and Geography, Bangor University.

In the laboratory soil cores were divided into depth intervals of 0-15, 15-30, 50-100, 100-150, 150-200 and 250-300 centimetres and passed through a 5 millimetre sieve in order to remove stones and any plant material and to ensure sample homogeneity.

To measure the rate of 14C-substrate mineralization, 5 grams of soil (dry weight equivalent to account for soil water content variability down the soil profile) was placed into sterile 50ml polypropylene tubes. To determine the rate of 14CO2 evolution 160µl of the recalcitrant 14C-DOC labelled nutrient solution was added to the soil surface. Immediately after nutrient addition, a vial containing 1 millilitre (ml) Sodium Hydroxide (NaOH) trap (1 Molar (M)) was added into the polypropylene tubes to capture 14CO2 evolved. The tubes were hermetically sealed and incubated at 10 degrees Celsius to represent the mean annual temperature of the catchment. The NaOH traps were changed at 1, 6, 24, 48, 72, 168, 336, 504, 672, 840, 1176, 1512 and 1680 hours. On removal, the NaOH traps were mixed with Optiphase HiSafe 3® liquid scintillation fluid (PerkinElmer Inc.) and the amount of 14CO2 captured was determined using a Wallac 1404 liquid scintillation counter (Wallac EG & G).

Data were entered into an Excel spreadsheet and exported as a .csv file for ingestion into the EIDC.

Metadata

File identifier
4a8fedf4-a572-4d95-ac41-087974169af1 XML
Metadata Language
English (en)
Character set
ISO/IEC 8859-1 (also known as Latin 1)
Resource type
dataset
Hierarchy level name
dataset
Metadata Date
2022-05-20T10:41:59
Metadata standard name
UK GEMINI
Metadata standard version
2.3
  Environmental Information Data Centre
Lancaster Environment Centre, Library Avenue, Bailrigg , Lancaster , LA1 4AP , UK
 
 

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