Population dynamics of evolving bacterial populations in liquid media, 2020

This data was collected from co-evolving bacterial populations containing Pseudomonas fluorescens strain SBW25 and a plasmid, pQBR57. The composition of the community was tracked using flow cytometry to distinguish 1) an unlabelled wild type strain 2) a dTomato compensated host (SBW25 KO PFLU4242), and 3) a wild type host bearing a Green fluorescent protein (GFP) labelled compensated plasmid (pQBR57 KO 0059). Full details about this dataset can be found at https://doi.org/10.5285/51046841-deaa-422f-a303-2c0759f014b4

Identification info

Metadata Language: English (en)

Character set: utf8

Dataset Reference Date (): 2022-11-23

Identifier: https://catalogue.ceh.ac.uk/id/51046841-deaa-422f-a303-2c0759f014b4

Identifier: doi: / 10.5285/51046841-deaa-422f-a303-2c0759f014b4

Other citation details: Wright, R. (2022). Population dynamics of evolving bacterial populations in liquid media, 2020. NERC EDS Environmental Information Data Centre 10.5285/51046841-deaa-422f-a303-2c0759f014b4

University of Manchester - Wright, R.

NERC EDS Environmental Information Data Centre

University of Manchester

GEMET - INSPIRE themes, version 1.0 ()

Environmental Monitoring Facilities

Keywords

co-evolution
plasmid compensation
SBW25
pQBR57

Limitations on Public Access: otherRestrictions

Other constraints: no limitations

Use constraints: otherRestrictions

Other constraints: This resource is available under the terms of the Open Government Licence

Use constraints: otherRestrictions

Other constraints: If you reuse this data, you should cite: Wright, R. (2022). Population dynamics of evolving bacterial populations in liquid media, 2020. NERC EDS Environmental Information Data Centre https://doi.org/10.5285/51046841-deaa-422f-a303-2c0759f014b4

Spatial representation type: textTable

Topic category

Biota

Begin date: 2020-01-01

End date: 2020-12-31

Code: OSGB 1936 / British National Grid

Distribution Information

Data format

text ()

Resource Locator: Download the data
Download a copy of this data

Resource Locator: Supporting information
Supporting information available to assist in re-use of this dataset

Resource Locator: Download the data
Download a copy of this data

Resource Locator: Supporting information
Supporting information available to assist in re-use of this dataset

Quality Scope: dataset

Other: dataset

Report

Dataset Reference Date (): 2010-12-08

Statement: Flow cytometry data was filtered to remove background noise (using thresholds in forward scatter (FSC-H), side scatter (SSC-H) and blue signal (PB450-H) channels), then all events were classified by fluorescence (flow_data.txt) to provide a community composition for each sample recorded as population fractions (fraction_data.txt).

Metadata

File identifier: 51046841-deaa-422f-a303-2c0759f014b4 XML

Metadata Language: English (en)

Character set: ISO/IEC 8859-1 (also known as Latin 1)

Resource type: dataset

Hierarchy level name: dataset

Metadata Date: 2022-11-25T11:18:32

Metadata standard name: UK GEMINI

Metadata standard version: 2.3

Environmental Information Data Centre

Lancaster Environment Centre, Library Avenue, Bailrigg , Lancaster , LA1 4AP , UK

Overviews

Publishing Body

Access the portal

Full access to the portal and metadata.

Associated resources (if any)

Not available

Default