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Flow cytometry analysis of water samples for bacterial and Pico-plankton enumeration, samples collected in the Barents Sea during 2017-2018

Collection and preservation of open ocean water samples from stations along a transect in the Barents Sea over the course of a year from July 2017 - July 2018. Four cruises in total to cover seasonal changes, two on board the James Clark Ross (RRS) and two aboard the Helmer Hansen (RV). A standard CTD cast was deployed to collect the samples, the depths were selected to support Primary Production experiments on board the ship, with deep samples representing 1% PAR. Research assistants from SAMS (Scottish Association for Marine Science) were responsible for the sample collection and Elaine Mitchell of SAMS was responsible for the sample analysis and data processing.

This work was funded by Arctic PRIZE - NERC Thematic grant - Changing Arctic Ocean (CAO) programme - NE/P006302/1.

Simple

Alternate title
Polar Data Centre (PDC) record GB/NERC/BAS/PDC/01300
Date (Publication)
2022-02-03
Identifier
http://www.antarctica.ac.uk/dms/metadata.php?id= / GB/NERC/BAS/PDC/01300
Custodian
  British Antarctic Survey
High Cross, Madingley Road , Cambridge , CB3 0ET , UK
+44 (0)1223 221400
Originator
  Polar Data Centre,Natural Environment Research Council,UK Research & Innovation - Mitchell, Elaine;McNeil, Sharon;Whyte, Callum;Cottier, Finlo;Hopkins, Joanne;Davidson, Keith
High Cross, Madingley , Cambridge , CB3 0ET , UK
+44 (0)1223 221400
Maintenance and update frequency
unknown Unknown
Keywords
  • NDGO0001
NERC OAI Harvesting
  • NERC_DDC
GCMD Parameter Valids
  • EARTH SCIENCE > Biosphere > Ecological Dynamics > Biomass
  • EARTH SCIENCE > Biosphere > Microbiota Taxonomy > Bacteria
  • EARTH SCIENCE > Biosphere > Microbiota Taxonomy > Plankton
  • EARTH SCIENCE > Oceans > Marine Environment Monitoring
BAS Free-text keywords
  • Bacteria
  • Barents Sea
  • Biomass
  • Flow cytometry
  • Pico-plankton
Use limitation
Dataset is released under Open Government Licence V3.0: http://www.nationalarchives.gov.uk/doc/open-government-licence/version/3/.
Access constraints
otherRestrictions Other restrictions
Other constraints
Dataset is released under Open Government Licence V3.0: http://www.nationalarchives.gov.uk/doc/open-government-licence/version/3/.
Metadata language
EnglishEnglish
Topic category
  • Biota
  • Environment
  • Oceans
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Begin date
2018-01-08
End date
2018-01-16
Reference system identifier
OGP / urn:ogc:def:crs:EPSG::4326
Distribution format
Distributor
  Polar Data Centre - British Antarctic Survey
+44 (0)1223 221400
OnLine resource
https://data.bas.ac.uk/metadata.php?id=GB/NERC/BAS/PDC/01167
Hierarchy level
dataset Dataset

Domain consistency

Measure identification
INSPIRE / Conformity_001

Conformance result

Date
Explanation
See the referenced specification
Pass
No
Statement

Setting up of the gates for the three sets of cell analysis was done by hand for each sample run by Elaine Mitchell. Samples for flow cytometry really should be done fresh without freezing, as freezing does cause damage to the cells which in turn can create indistinct dot plots. Gating these plots is tricky but by analysing the samples using a range of plots with different parameters and logic gates this should have reduced any errors to a minimum.

Mike Zubkov at SAMS checked the data for errors/alterations before submission.

File identifier
GB_NERC_BAS_PDC_01300 XML
Metadata language
EnglishEnglish
Hierarchy level
dataset Dataset
Date stamp
2022-02-03
Metadata standard name
NERC profile of ISO19115:2003
Metadata standard version
1.0
Point of contact
  British Antarctic Survey
+44 (0)1223 221400
Dataset URI
http://www.antarctica.ac.uk/dms/metadata.php?id=GB/NERC/BAS/PDC/01300
 
 

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Keywords


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