NERC Data Catalogue Service
Flow cytometry analysis of water samples for bacterial and Pico-plankton enumeration, samples collected in the Barents Sea during 2017-2018
Collection and preservation of open ocean water samples from stations along a transect in the Barents Sea over the course of a year from July 2017 - July 2018. Four cruises in total to cover seasonal changes, two on board the James Clark Ross (RRS) and two aboard the Helmer Hansen (RV). A standard CTD cast was deployed to collect the samples, the depths were selected to support Primary Production experiments on board the ship, with deep samples representing 1% PAR. Research assistants from SAMS (Scottish Association for Marine Science) were responsible for the sample collection and Elaine Mitchell of SAMS was responsible for the sample analysis and data processing.
This work was funded by Arctic PRIZE - NERC Thematic grant - Changing Arctic Ocean (CAO) programme - NE/P006302/1.
Simple
- Alternate title
- Polar Data Centre (PDC) record GB/NERC/BAS/PDC/01300
- Date (Publication)
- 2022-02-03
- Identifier
- http://www.antarctica.ac.uk/dms/metadata.php?id= / GB/NERC/BAS/PDC/01300
- Maintenance and update frequency
- unknown Unknown
- Keywords
-
- NDGO0001
- NERC OAI Harvesting
-
- NERC_DDC
- GCMD Parameter Valids
-
- EARTH SCIENCE > Biosphere > Ecological Dynamics > Biomass
- EARTH SCIENCE > Biosphere > Microbiota Taxonomy > Bacteria
- EARTH SCIENCE > Biosphere > Microbiota Taxonomy > Plankton
- EARTH SCIENCE > Oceans > Marine Environment Monitoring
- BAS Free-text keywords
-
- Bacteria
- Barents Sea
- Biomass
- Flow cytometry
- Pico-plankton
- Use limitation
- Dataset is released under Open Government Licence V3.0: http://www.nationalarchives.gov.uk/doc/open-government-licence/version/3/.
- Access constraints
- otherRestrictions Other restrictions
- Other constraints
- Dataset is released under Open Government Licence V3.0: http://www.nationalarchives.gov.uk/doc/open-government-licence/version/3/.
- Metadata language
- EnglishEnglish
- Topic category
-
- Biota
- Environment
- Oceans
))
- Begin date
- 2018-01-08
- End date
- 2018-01-16
- Reference system identifier
- OGP / urn:ogc:def:crs:EPSG::4326
- Distribution format
-
- OnLine resource
- https://data.bas.ac.uk/metadata.php?id=GB/NERC/BAS/PDC/01167
- Hierarchy level
- dataset Dataset
Domain consistency
- Measure identification
- INSPIRE / Conformity_001
Conformance result
- Date
- Explanation
- See the referenced specification
- Pass
- No
- Statement
-
Setting up of the gates for the three sets of cell analysis was done by hand for each sample run by Elaine Mitchell. Samples for flow cytometry really should be done fresh without freezing, as freezing does cause damage to the cells which in turn can create indistinct dot plots. Gating these plots is tricky but by analysing the samples using a range of plots with different parameters and logic gates this should have reduced any errors to a minimum.
Mike Zubkov at SAMS checked the data for errors/alterations before submission.
- File identifier
- GB_NERC_BAS_PDC_01300 XML
- Metadata language
- EnglishEnglish
- Hierarchy level
- dataset Dataset
- Date stamp
- 2022-02-03
- Metadata standard name
- NERC profile of ISO19115:2003
- Metadata standard version
- 1.0
Overviews
Spatial extent
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