Nitrous oxide emissions and associated microbial diversity, soil biochemical properties and crop growth and yield from a field trial of winter barley with the addition of microplastics, Abergwyngregyn, UK, 2020-2021
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- Metadata Language
- English (en)
- Character set
- utf8
- Dataset Reference Date ()
- 2022-03-02
- Identifier
- doi: / 10.5285/a5410834-1c38-455b-a850-3fb3434d4bb0
- Other citation details
- Greenfield, L.M., Graf, M., Rengaraj, S., Bargiela, R., Williams, G.B. , Golyshin, P.N., Chadwick, D.R., Jones, D.L. (2022). Nitrous oxide emissions and associated microbial diversity, soil biochemical properties and crop growth and yield from a field trial of winter barley with the addition of microplastics, Abergwyngregyn, UK, 2020-2021. NERC EDS Environmental Information Data Centre 10.5285/a5410834-1c38-455b-a850-3fb3434d4bb0
- Maintenance and update frequency
- notPlanned
- GEMET - INSPIRE themes, version 1.0 ()
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- Environmental Monitoring Facilities
- Limitations on Public Access
- otherRestrictions
- Other constraints
- no limitations
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- If you reuse this data, you should cite: Greenfield, L.M., Graf, M., Rengaraj, S., Bargiela, R., Williams, G.B. , Golyshin, P.N., Chadwick, D.R., Jones, D.L. (2022). Nitrous oxide emissions and associated microbial diversity, soil biochemical properties and crop growth and yield from a field trial of winter barley with the addition of microplastics, Abergwyngregyn, UK, 2020-2021. NERC EDS Environmental Information Data Centre https://doi.org/10.5285/a5410834-1c38-455b-a850-3fb3434d4bb0
- Spatial representation type
- textTable
- Distance
- 12 urn:ogc:def:uom:EPSG::9001
- Topic category
-
- Biota
- Environment
- Begin date
- 2020-09-01
- End date
- 2021-07-31
- Code
- WGS 84
Distribution Information
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Comma-separated values (CSV)
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Comma-separated values (CSV)
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- Quality Scope
- dataset
- Other
- dataset
Report
- Dataset Reference Date ()
- 2010-12-08
- Statement
- Data was either measured in situ in the field (N2O flux, soil moisture, rainfall and air temperature) or samples were taken, processed, and analysed in the laboratory (soil pH, electrical conductivity (EC), ammonium, nitrate, microbial community composition and crop yield). N2O flux data was measured on a mobile gas chromatograph (GC) system and integrated to obtain peak areas on Peak490Win10Canabis programme. The times, peak areas and sample ID were then exported into a .CHR file and imported into Flux.NET.3.3 which calculated N2O flux as an output in Excel which was exported as .csv file for deposit in EIDC. N2O flux was used to calculate cumulative N2O flux using trapezoidal integration in Excel and saved in a separate .csv file for deposit in EIDC. Soil moisture was measured on Accilmas with data stored as a .csv on a DataSnap that was downloaded and sorted by treatment and saved as a .csv file. Rainfall and air temperature were downloaded from the weather station as .csv file. Soil pH and EC were recorded manually into a notebook and input into an Excel spreadsheet and exported as a .csv file. Soil ammonium and nitrate content was measured using the microplate method using a programme called Gen5. Date was exported into an Excel spreadsheet and absorbance units used to calculate ammonium/nitrate content in milligrams per kilogram using a calibration curve from a set of standards in an Excel spreadsheet. This was exported as a .csv file. Crop growth data was recorded in the field in a notebook and input into an Excel spreadsheet and exported as a .csv file. Crop yield was recorded in a notebook and input into an Excel spreadsheet and exported as a .csv file. Microbial community composition was measured using 16S gene sequencing on an Illumina MiSeq. This generated raw sequencing reads which were processed using Python and filtered using QIIME v1.3.1. creating asv.count.table.csv of counts of each Amplicon Sequence Variants (ASVs) per sample and taxa.table.csv of the taxonomic lineage for each ASVs.
Metadata
- File identifier
- a5410834-1c38-455b-a850-3fb3434d4bb0 XML
- Metadata Language
- English (en)
- Character set
- ISO/IEC 8859-1 (also known as Latin 1)
- Resource type
- dataset
- Hierarchy level name
- dataset
- Metadata Date
- 2024-02-13T09:30:58
- Metadata standard name
- UK GEMINI
- Metadata standard version
- 2.3