Molecular analysis of Glossina morsitans morsitans tsetse from Mambwe District, Zambia (2013)

This data set describes the prevalence of trypanosomes and Sodalis glossinidius, and host blood meal analysis from tsetse flies (Glossina morsitans morsitans) captured during two intensive surveys in Mambwe District, Eastern Province, Zambia in 2013. The Luangwa Valley in Zambia is an old focus of human African trypanosomiasis (HAT, Trypanosoma brucei rhodesiense) and sporadic outbreaks have continued to occur in the human population. In recent years there has been an influx of people migrating from the densely populated plateau region resulting in a significant change in land-use in the study area, potentially influencing tsetse dynamics and the epidemiology of HAT. This data set was collected to monitor infection rates of trypanosomes and Sodalis glossinidius in Glossina morsitans morsitans tsetse flies in the area so as to assess the risk posed to both human and livestock populations. In addition, feeding patterns of tsetse were investigated through analysis of blood meals.

This work was part of a wider research project, the Dynamic Drivers of Disease in Africa Consortium (DDDAC) and contributed to the Zambia trypanosomiasis case study. The research was funded by NERC project no NE/J000701/1 with support from the Ecosystem Services for Poverty Alleviation Programme (ESPA).

Full details about this dataset can be found at https://doi.org/10.5285/a55eea77-8401-49ba-921e-53e085dc8345

Identification info

Metadata Language: English (en)

Character set: utf8

Dataset Reference Date (): 2017-03-24

Dataset Reference Date (): 2016-11-21

Identifier: https://catalogue.ceh.ac.uk/id/a55eea77-8401-49ba-921e-53e085dc8345

Identifier: doi: / 10.5285/a55eea77-8401-49ba-921e-53e085dc8345

Other citation details: Simuunza, M., Anderson, N.E, Machila, N., Ndebe, J. , Macleod, E.T., Welburn, S.C. (2017). Molecular analysis of Glossina morsitans morsitans tsetse from Mambwe District, Zambia (2013). NERC Environmental Information Data Centre 10.5285/a55eea77-8401-49ba-921e-53e085dc8345

University of Zambia - Simuunza, M.

The University of Edinburgh - Anderson, N.E

University of Zambia - Simuunza, M.

The University of Edinburgh - Anderson, N.E

University of Zambia - Machila, N.

University of Zambia - Ndebe, J.

The University of Edinburgh - Macleod, E.T.

The University of Edinburgh - Welburn, S.C.

NERC EDS Environmental Information Data Centre

NERC Environmental Information Data Centre

Wikidata

GeoNames

Zambia

Keywords

Blood meal
Tsetse
trypanosome
Dynamic Drivers of Disease in Africa Consortium (DDDAC)
Luangwa Valley

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Other constraints: This resource is made available under the terms of the Open Government Licence

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Other constraints: © The University of Edinburgh

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Other constraints: © University of Zambia

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Other constraints: If you reuse this data, you should cite: Simuunza, M., Anderson, N.E, Machila, N., Ndebe, J. , Macleod, E.T., Welburn, S.C. (2017). Molecular analysis of Glossina morsitans morsitans tsetse from Mambwe District, Zambia (2013). NERC Environmental Information Data Centre https://doi.org/10.5285/a55eea77-8401-49ba-921e-53e085dc8345

Spatial representation type: textTable

Topic category

Biota
Health

Begin date: 2013-05-01

End date: 2013-11-30

Code: WGS 84

Distribution Information

Data format

Comma-separated values (CSV) ()

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Quality Scope: dataset

Other: dataset

Report

Dataset Reference Date (): 2010-12-08

Statement: Tsetse surveys were conducted in May / June 2013 and November 2013. Tsetse flies were sampled using black screen fly rounds. Data were recorded on standard field sampling forms and then manually transferred to Microsoft Excel. Data were then checked for accuracy and incorrect spatial location coordinates removed.

Tsetse flies were homogenized and DNA extracted. The resulting DNA was stored at minus 20 degrees Celsius until needed for Polymerase chain reaction (PCR). PCR analysis was performed using different primers to detect the different species of trypanosomes in the DNA. All PCR included a negative and a positive control.

To determine the sources of blood meal (feeding patterns), tsetse DNA samples were subjected to PCR targeting the cytochrome oxidase b gene. The resulting PCR product was sequenced, and the sequences compared with those available in public databases by using nucleotide BLAST at NCBI website (http://blast.ncbi.nlm.nih.gov/Blast.cgi) to determine the animal species on which tsetse fed on. Data were collated in a Microsoft Excel spreadsheet and exported as a comma separated file for ingestion into the Environmental Information Data Centre (EIDC).

Metadata

File identifier: a55eea77-8401-49ba-921e-53e085dc8345 XML

Metadata Language: English (en)

Character set: ISO/IEC 8859-1 (also known as Latin 1)

Resource type: dataset

Hierarchy level name: dataset

Metadata Date: 2021-10-06T14:50:21

Metadata standard name: UK GEMINI

Metadata standard version: 2.3

Environmental Information Data Centre

Lancaster Environment Centre, Library Avenue, Bailrigg , Lancaster , LA1 4AP , UK

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