Soil microbial potential enzyme activities in fertilised plots in the Central Amazon, 2018

The data consists of potential activity of enzymes released by microorganisms in soils of old growth forests in the Central Amazon. Soils were sampled in January 2018, seven months after nutrient addition in a large-scale nutrient fertilisation experiment. Enzyme activities are given as a mean of the microbial community per plot, where five points inside each plot were sampled in the 0-5 cm and 5-10 cm soil layers. The dataset depicts the potential activities of the following enzymes: ß-glucosidase, N-acetyl ß-glucosaminidase and phosphomonoesterase at two different soil depths.

Samples were collected at the AFEX project area in Manaus, Brazil at the Biological Dynamics of Forest Fragments Project (BDFFP/ INPA).

The study was funded by NERC, BDFFP (logistical support) and Brazilian government (student scholarship). Full details about this dataset can be found at https://doi.org/10.5285/b9a2523e-09ba-43c7-acfa-a894a231b133

Identification info

Metadata Language: English (en)

Character set: utf8

Dataset Reference Date (): 2020-04-02

Identifier: https://catalogue.ceh.ac.uk/id/b9a2523e-09ba-43c7-acfa-a894a231b133

Identifier: doi: / 10.5285/b9a2523e-09ba-43c7-acfa-a894a231b133

Other citation details: Lugli, L.F., Quesada, C.A.N., Andersen, K.M., Mercado, L. M., Di Ponzio, R., Ribeiro, G., Assis, R. L. , Hartley, I.P. (2020). Soil microbial potential enzyme activities in fertilised plots in the Central Amazon, 2018. NERC Environmental Information Data Centre 10.5285/b9a2523e-09ba-43c7-acfa-a894a231b133

National Institute of Amazonian Research, Brazil - Lugli, L.F.

National Institute of Amazonian Research, Brazil - Quesada, C.A.N.

Nanyang Technological University, Singapore - Andersen, K.M.

University of Exeter - Mercado, L. M.

National Institute of Amazonian Research, Brazil - Di Ponzio, R.

National Institute of Amazonian Research, Brazil - Ribeiro, G.

Natural History Museum, University of Oslo, Norway - Assis, R. L.

University of Exeter - Hartley, I.P.

National Institute of Amazonian Research, Brazil - Laynara Lugli

University of Exeter - Prof. Iain Hartley

NERC Environmental Information Data Centre

NERC EDS Environmental Information Data Centre

GEMET - INSPIRE themes, version 1.0 ()

Soil

Keywords

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Other constraints: © Natural Environment Research Council

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Other constraints: This resource is available under the terms of the Open Government Licence

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Other constraints: If you reuse this data, you should cite: Lugli, L.F., Quesada, C.A.N., Andersen, K.M., Mercado, L. M., Di Ponzio, R., Ribeiro, G., Assis, R. L. , Hartley, I.P. (2020). Soil microbial potential enzyme activities in fertilised plots in the Central Amazon, 2018. NERC Environmental Information Data Centre https://doi.org/10.5285/b9a2523e-09ba-43c7-acfa-a894a231b133

Spatial representation type: textTable

Distance: 1000 urn:ogc:def:uom:EPSG::9001

Topic category

Biota
Environment

Begin date: 2018-01-01

End date: 2018-09-30

Code: WGS 84

Distribution Information

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Comma-separated values (CSV) ()

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Report

Dataset Reference Date (): 2010-12-08

Statement: Potential activities of 4-Methylumbelliferyl-β-D-glucopyranoside (BG), 4-Methylumbelliferyl-N-acetyl-β-D-glucosaminide (NAG) and 4-Methylumbelliferyl-phosphate (PHOS), enzymes involved in the cycling of carbon, nitrogen and phosphorus were measured using microplate fluorimetric assays. After centrifugation with buffer solution, samples were incubated for one hour using 4-Methylumbelliferyl-β-D-glucopyranoside (BG), 4-Methylumbelliferyl-N-acetyl-β-D-glucosaminide (NAG) and 4-Methylumbelliferyl-phosphate (PHOS) as substrates. Fluorescence was read on a fluorometer (Tecan Infinite® 200 PRO, Grödig, Austria), at 365 nm excitation and 450 nm emission. Enzyme activities are presented in nmol MUF g soil dry weight-1 hour-1.

Metadata

File identifier: b9a2523e-09ba-43c7-acfa-a894a231b133 XML

Metadata Language: English (en)

Character set: ISO/IEC 8859-1 (also known as Latin 1)

Resource type: dataset

Hierarchy level name: dataset

Metadata Date: 2022-05-18T13:26:09

Metadata standard name: UK GEMINI

Metadata standard version: 2.3

Environmental Information Data Centre

Lancaster Environment Centre, Library Avenue, Bailrigg , Lancaster , LA1 4AP , UK

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