NERC Data Catalogue Service
Soil fungi gene copy numbers and community composition under birch and willow vegetation in girdled and non-girdled plots, subarctic Sweden, 2017-2019
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Identification info
- Metadata Language
- English (en)
- Character set
- utf8
- Dataset Reference Date ()
- 2022-08-31
- Identifier
- doi: / 10.5285/d6c787ec-146c-461b-b8a2-e0251259036c
- Other citation details
- Parker, T.C., Chomel, M., Clemmensen, K.E., Friggens, N.L., Hartley, I.P., Johnson, D., Krab, E.J., Lindahl, B.D., Street, L.E., Subke, J.-A., Wookey, P.A. (2022). Soil fungi gene copy numbers and community composition under birch and willow vegetation in girdled and non-girdled plots, subarctic Sweden, 2017-2019. NERC EDS Environmental Information Data Centre 10.5285/d6c787ec-146c-461b-b8a2-e0251259036c
- GEMET - INSPIRE themes, version 1.0 ()
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- Environmental Monitoring Facilities
- Keywords
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- Subarctic
- Treeline
- Tundra
- Soil funngi
- Girdling
- Limitations on Public Access
- otherRestrictions
- Other constraints
- no limitations
- Use constraints
- otherRestrictions
- Use constraints
- otherRestrictions
- Other constraints
- If you reuse this data, you should cite: Parker, T.C., Chomel, M., Clemmensen, K.E., Friggens, N.L., Hartley, I.P., Johnson, D., Krab, E.J., Lindahl, B.D., Street, L.E., Subke, J.-A., Wookey, P.A. (2022). Soil fungi gene copy numbers and community composition under birch and willow vegetation in girdled and non-girdled plots, subarctic Sweden, 2017-2019. NERC EDS Environmental Information Data Centre https://doi.org/10.5285/d6c787ec-146c-461b-b8a2-e0251259036c
- Spatial representation type
- textTable
- Distance
- 3 urn:ogc:def:uom:EPSG::9001
- Topic category
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- Biota
- Environment
))
- Begin date
- 2017-08-03
- End date
- 2019-08-05
Distribution Information
- Data format
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Comma-separated values (CSV)
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Comma-separated values (CSV)
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Supporting information
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- Quality Scope
- dataset
- Other
- dataset
Report
- Dataset Reference Date ()
- 2010-12-08
- Statement
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The organic soil horizon (O horizon) was sampled on 3rd August 2017, 1st August 2018 and 5th August 2019. For each plot (22 in total), nine 3.8 cm diameter soil cores were collected; in the birch plots, cores were taken in a grid across a 3 x 3 m central area (corresponding to the central area in which soil CO2 efflux, root and mycelium production and soil fauna measurements were taken; (Parker et al., 2020). In the willow plots, the nine cores were distributed evenly across the plot area within the trenched perimeter. The uppermost litter layer, as well as the mineral horizons underlying the organic horizon (clearly identifiable at these sites), were removed from cores immediately after coring. The nine cores from each plot were homogenised and pooled within 6 hours of sampling.
DNA was extracted from a 50 mg sub-sample and ITS2 markers were amplified by PCR. We used a community metabarcoding approach on the pooled DNA samples to identify distinct taxonomic groups at species level where possible. Copy numbers of the fungal ITS2 region were estimated by quantitative PCR (qPCR)
Metadata
- File identifier
- d6c787ec-146c-461b-b8a2-e0251259036c XML
- Metadata Language
- English (en)
- Character set
- ISO/IEC 8859-1 (also known as Latin 1)
- Resource type
- dataset
- Hierarchy level name
- dataset
- Metadata Date
- 2022-09-05T10:47:46
- Metadata standard name
- UK GEMINI
- Metadata standard version
- 2.3
