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  • This dataset contains bird and butterfly abundance data from field-surveyed transects at Hillesden, UK (2006-2017). Over this time period, Hillesden hosted two five-year experimental manipulations of agri-environmental habitats, alongside monitoring of their impacts on biodiversity and agronomic indicators. This dataset contains data suitable for analysis of ten-year interannual population trends across both 5-year phases of experimental work at Hillesden, and subsequent comparison with equivalent trends derived from equivalent national recording schemes (Breeding Bird Survey (BBS) for birds, Wider Countryside Butterfly Monitoring Scheme (WCBMS) for butterflies). The data thus relate only to transects which were recorded consistently (nine for birds, seven for butterflies), and to species that were present on the majority of transects. This dataset thus represents a subset from the whole Hillesden biological survey dataset for these two groups, and contains maximum annual counts per transect, across monthly visits (April-August) within the permitted time frame from equivalent national recording schemes (BBS for birds, WCBMS for butterflies). Data were collected as part of a project led by the UK Centre for Ecology and Hydrology, funded by Defra, with analytical work funded by the Natural Environment Research Council (NERC) under research programme NE/N018125/1 ASSIST. ASSIST is an initiative jointly supported by NERC and the Biotechnology and Biological Sciences Research Council (BBSRC). Full details about this dataset can be found at

  • The dataset contains measurements of fluorescein, dopachrome and nitrous oxide production from incubation experiments of streambed sediments. The sediments were collected in five pseudo-replicates from 0 to 10cm depth using a 5cm-diameter AMS slide hammer and trowel at two locations within the Wood Brook, Staffordshire, in June 2015. Samples were collected from two reaches within the stream, a mid-stream sandy reach and a downstream gravel reach. The sediment was used in laboratory incubation experiments to determine rates of fluorescein diacetate (FDA) hydrolysis (total microbial activity), extracellular phenol oxidase activity (recalcitrant carbon uptake) and potential denitrification from sand versus gravel sediments. Also included is the organic matter content of the sediments. The work was funded by the Natural Environment Research Council. Full details about this dataset can be found at

  • The dataset contains chemistry data from streambed porewater (10 and 20 cm) and surface water, as well as nitrogen chemistry data at 2.5 cm resolution within the upper 15 cm of the streambed. The dataset includes concentrations of dissolved organic carbon (DOC), carbon dioxide, methane, ammonium, nitrate, nitrite and nitrous oxide, and isotopic ratios of δ13CCO2, δ15NNO3+NO2 and δ18ONO3+NO2. Also included are measurements of dissolved oxygen and temperature. Samples were collected from three reaches within the stream, an upstream sandy reach, a mid-stream sandy reach and a downstream gravel reach. The work was carried out with Natural Environment Research Council (NERC) funding through a PhD (NERC award number 1602135), grant (NE/L004437/1) and Life Sciences Mass Spectrometry Facility grant (CEH_L102_05_2016). Full details about this dataset can be found at