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Soil temperature, soil moisture, air temperature and relative humidity for vegetation at Siksik Creek, North West Territories, Canada
Data comprise soil temperature, air temperature, soil volumetric moisture content, relative humidity, and surface wetness data from Onset Microstation Data Loggers at 5 locations (within the main vegetation types) at SikSik creek catchment, Trail Valley Creek, NWT, Canada. The data were collected under Project HYDRA, a NERC funded UK research project linking Heriot Watt University, the Universities of Durham, Aberdeen and Stirling, and the Centre for Ecology & Hydrology (CEH), Edinburgh. Project HYDRA is part of the UK Arctic Research Programme. Project HYDRA studies sites in Arctic Canada to investigate the biological, chemical and physical controls on the release of greenhouse gases from permafrost into melt water and to the atmosphere and how these emissions will influence global warming. Full details about this dataset can be found at https://doi.org/10.5285/10839b38-cc29-4a07-999a-ac32e3f70609
DNA fingerprints to determine self-fertilization (homothallism) or outcrossing (heterothallism) in Antarctic lichen
The majority of Antarctic lichens produce sexual organs, and in many species sexual ascospores appear to be the only reproductive propagule. However, it is unknown whether sexual reproduction involves selfing (homothallism) or outcrossing (heterothallism). To investigate this issue we have established axenic cultures of sexual progeny in order to generate DNA fingerprints and thereby determine the breeding system.
Genetic variation on a spatial scale was assessed, using both DNA fingerprinting and sequencing-based approaches, in the Antarctic endemics Buellia frigida, Carbonia vorticosa and Amandinea petermananii, and in the bipolar species Caloplaca saxicola, Umbilicaria decussata and Cladonia galindezii. PCR-based (Polymerase Chain Reaction) molecular biology techniques, were used as they are ideal for working with lichens because little starting material is required. See Fabian et al. 2007 for further information on analyses and results.
Baseline Vegetation data from the UK Environmental Change Network (ECN) terrestrial sites. These data are collected at all of ECN's terrestrial sites using a standard protocol (see supporting documentation). This was a one-off whole site baseline vegetation survey (though given the intensive nature of this survey, some sites did it over successive years) to generate a vegetation map and identify the areas within the site to be monitored. In this protocol up to 500 systemic 2m x 2m plots were surveyed and species presence recorded. ECN is the UK's long-term environmental monitoring programme. It is a multi-agency programme sponsored by a consortium of fourteen government departments and agencies. These organisations contribute to the programme through funding either site monitoring and/or network co-ordination activities. These organisations are: Agri-Food and Biosciences Institute, Biotechnology and Biological Sciences Research Council, Cyfoeth Naturiol Cymru - Natural Resources Wales, Defence Science & Technology Laboratory, Department for Environment, Food and Rural Affairs, Environment Agency, Forestry Commission, Llywodraeth Cymru - Welsh Government, Natural England, Natural Environment Research Council, Northern Ireland Environment Agency, Scottish Environment Protection Agency, Scottish Government and Scottish Natural Heritage. Full details about this dataset can be found at https://doi.org/10.5285/a7b49ac1-24f5-406e-ac8f-3d05fb583e3b
This database contains information on the herbarium specimens held in the herbarium of the British Antarctic Survey (international code AAS) as well as information about specimens collected in the Antarctic and sub-Antarctic and held in other world herbaria. There are over 70 000 records, predominantly of mosses and lichens, but also of vascular plants, ferns, fungi and algae collected in Antarctic and sub-Antarctic regions as well as some from surrounding continents, particularly South America. The collection from South Georgia And The South Sandwich Islands started in 1775 and from Antarctica in 1834. Documents relating to the Herbarium are kept in the BAS Archives (LS2/4). The records can be searched and downloaded on: http://apex.nerc-bas.ac.uk/f?p=148:1. There is also a facility to see a distribution map of specimens retrieved by querying the database.
The fieldwork involved collection of fertile lichens from a range of sites across the Antarctic Peninsula and isolation of the lichen-forming fungi into pure culture in a laboratory at Rothera. Approximately 5,600 monospore cultures were isolated, including B frigida. Approximately 400 thalli of Usnea species, and 3 O. frigida thalli have also been collected for whole thallus analysis. Logarithmic sampling transects of B frigida were conducted at Rothera (2 transects) and on Anchorage Island (one transect) to examine the genetic variation and geographic variation. All thalli of B frigida collected from the transects were successfully used to generate viable spores from four individual apothecia from each thallus. 16 spores were subcultured and maintained from each apothecium.