The dataset contains chemistry data from streambed porewater (10 and 20 cm) and surface water, as well as nitrogen chemistry data at 2.5 cm resolution within the upper 15 cm of the streambed. The dataset includes concentrations of dissolved organic carbon (DOC), carbon dioxide, methane, ammonium, nitrate, nitrite and nitrous oxide, and isotopic ratios of δ13CCO2, δ15NNO3+NO2 and δ18ONO3+NO2. Also included are measurements of dissolved oxygen and temperature. Samples were collected from three reaches within the stream, an upstream sandy reach, a mid-stream sandy reach and a downstream gravel reach. The work was carried out with Natural Environment Research Council (NERC) funding through a PhD (NERC award number 1602135), grant (NE/L004437/1) and Life Sciences Mass Spectrometry Facility grant (CEH_L102_05_2016). Full details about this dataset can be found at https://doi.org/10.5285/00601260-285e-4ffa-b381-340b51a7ec50

The dataset contains measurements of fluorescein, dopachrome and nitrous oxide production from incubation experiments of streambed sediments. The sediments were collected in five pseudo-replicates from 0 to 10cm depth using a 5cm-diameter AMS slide hammer and trowel at two locations within the Wood Brook, Staffordshire, in June 2015. Samples were collected from two reaches within the stream, a mid-stream sandy reach and a downstream gravel reach. The sediment was used in laboratory incubation experiments to determine rates of fluorescein diacetate (FDA) hydrolysis (total microbial activity), extracellular phenol oxidase activity (recalcitrant carbon uptake) and potential denitrification from sand versus gravel sediments. Also included is the organic matter content of the sediments. The work was funded by the Natural Environment Research Council. Full details about this dataset can be found at https://doi.org/10.5285/500193f7-2653-4696-8224-276a734ed6ab